Gwen Stacy: Gwen is torn between loyalty to her policeman father and her love for Peter, who is quickly becoming the bane of her father's existence. She also works part-time at Oscorp.

Dr. Connors: A former associate of Peter Parker's father, Connors is obsessed with cracking the code of reptile DNA, which allows lizards to re-grow limbs with ease. Could it allow him to regain the arm he lost?